MEMSuniverse » loading http://www.memsuniverse.com A Passion for Creativity & Innovation Tue, 23 Oct 2012 21:57:22 +0000 en-US hourly 1 http://wordpress.org/?v=3.4.2 cell trapping microfluidic device 2 http://www.memsuniverse.com/microfluidics/cell-trapping-microfluidic-device-2.html http://www.memsuniverse.com/microfluidics/cell-trapping-microfluidic-device-2.html#comments Sun, 25 Jan 2009 04:04:04 +0000 admin http://www.memsuniverse.com/?p=1014 cell loading experiment

Experimental loading of PDMS microfluidic array devices with 3T3 cells. Validation of the predictive value of modeling, using 3T3 cells flowed through microfluidic devices containing U-type sieves under the simulation flow parameters, showed excellent agreement between experiment and simulation with respect to cell
number per trap and the uniformity of cell distribution within individual microchambers. For applications such as on-chip cell culture or high-throughput screening of cell populations within a lab-on-a-chip environment

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701. 

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]]> http://www.memsuniverse.com/microfluidics/cell-trapping-microfluidic-device-2.html/feed 0 MFDEP cell loading device proof-of-concept http://www.memsuniverse.com/lab-on-chip/mfdep-cell-loading-device-proof-of-concept.html http://www.memsuniverse.com/lab-on-chip/mfdep-cell-loading-device-proof-of-concept.html#comments Mon, 14 Jan 2008 11:34:59 +0000 admin http://www.memsuniverse.com/?p=44 Proof-of-concept of a MEMS device that I designed for my PhD dissertation, at the University of Maryland. Cells are loaded inside a micro-cell cage using dielectrophoresis. The active cancellation of the parasitic traps is necesary, thus becoming the use of multiple frequencies (MFDEP). This is the first machine of its kind, and it is very useful if you need to load 1000′s of cells inside cages in matter of seconds. This technology is part of the “Cell Clinics” project (PI: Elisabeth Smela). The cells used are yeast cells. See the article entitled “Parasitic Trap Cancellation Using Multiple Frequency Dielectrophoresis, Demonstrated by Loading Cells into Cages”, upcomming in the journal Lab on a Chip.

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