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Using 3T3 cells as a model system, the motion of each individual cell was calculated using a one-way coupled Lagrangian method. The cell was assumed to be a solid sphere, and interactions with other cells were only considered when a cell sedimented in the trap. The ordinary differential equations were solved along the cell trajectory for the three components of the velocity and location vector by using the Rosenbrock method based on an adaptive time-stepping technique.

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701. 

This is an animation of Fig.1C in the paper published in J. Appl. Phys. 103, 044701 (2008).

Duration : 0:1:0

Experimental loading of PDMS microfluidic array devices with 3T3 cells. Validation of the predictive value of modeling, using 3T3 cells flowed through microfluidic devices containing U-type sieves under the simulation flow parameters, showed excellent agreement between experiment and simulation with respect to cell
number per trap and the uniformity of cell distribution within individual microchambers. For applications such as on-chip cell culture or high-throughput screening of cell populations within a lab-on-a-chip environment

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701. 

Duration : 0:0:26

The “flat-type sieve” consists of an array of nine sieves arranged in a symmetric diamond-shaped pattern. Under simulated conditions, sieves closer to the chamber entrances captured cells well for both geometries, while the downstream sieves remained empty.

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701

This is an animation of Fig. 1B in the paper published in J. Appl. Phys. 103, 044701 (2008).

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