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Microfluidic Encapsulation of Cells in Alginate Capsules for High Throughput Screening

Encapsulation of eukaryotic cells in alginate using commercially available microtubing. Liquid alginate droplets with encapsulated cells enter from the left. A crosslinking agent (barium chloride, stained with green dye for visualization) enters from the top. Hydrodynamic interactions in the T-junction adds a fixed amount of crosslinking agent to each droplet. Throughputs of up to 20 […]