Duration : 0:0:25

DNA Hybridization CD:

The final step in nucleic acid analysis is detection. Fluorescence is often used for detection, and specifically fluorescence microarrays are used when scanning for many elements at once. The Madou BioMEMS group had developed a modular, microfluidic DNA microarray/hybridization CD that allows for rapid hybridization and detection. DNA probes are spotted onto a glass slide using traditional methods, and a microfluidic PDMS unit is then bound passively to the slide. The slide-PDMS unit is then mounted in a CD holder. The sample is sent across the probe array, followed by a wash and then rinse. The entire process takes < 15 mins, a drastic improvement over traditional passive arrays. Finally, the PDMS unit is removed and the slide is scanned in a traditional fluorescence scanner. Superior signal to noise ratios are achieved, and specificity is excellent.

Publications:

Peytavi, R. (2005). Microfluidic Device for Rapid (< 15 min) Automated Microarray Hybridization. Clinical Chemistry, 51(10), 1836-.

Jia, G. (2006). Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform. Sensors and actuators. B, Chemical, 114(1), 173-.

Duration : 0:1:12

Using 3T3 cells as a model system, the motion of each individual cell was calculated using a one-way coupled Lagrangian method. The cell was assumed to be a solid sphere, and interactions with other cells were only considered when a cell sedimented in the trap. The ordinary differential equations were solved along the cell trajectory for the three components of the velocity and location vector by using the Rosenbrock method based on an adaptive time-stepping technique.

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701. 

This is an animation of Fig.1C in the paper published in J. Appl. Phys. 103, 044701 (2008).

Duration : 0:1:0

The “flat-type sieve” consists of an array of nine sieves arranged in a symmetric diamond-shaped pattern. Under simulated conditions, sieves closer to the chamber entrances captured cells well for both geometries, while the downstream sieves remained empty.

related article:
Building a Better Cell Trap: Applying Lagrangian Modeling to the Design of Microfluidic Devices for Cell Biology, Min-Cheol Kim, Zhanhui Wang, Raymond H. W. Lam and Todd Thorsen, Journal of Applied Physics, 103 (2008) 044701

This is an animation of Fig. 1B in the paper published in J. Appl. Phys. 103, 044701 (2008).

Duration : 0:1:0

Watch this strange unidentified object as it releases a translucent gel like hexagon from a crystal cup.
These strange microscopic objects were found in skin lesions of a person who suffers from both Lyme Disease and Morgellons. She also lives directly under a flight path of a major airport and near a 2001 hazmat event involving a (glycol) spill that required bioremediation.

I will be posting a few additional videos showing the objects glow (mirrors?) when in contact with metal; and have moving components (possibly nano. Please note the duel/split antennas and the fork tongue serpent on the back sides. Any info on this object is appreciated. If privacy is an issue email: chaosonline7@gmail.com

Duration : 0:1:33

I will be posting a few additional videos showing the objects glow (mirrors?) when in contact with metal; have moving components (possibly nano) and eject a translucent hexagon from a crystal like cup. Please note the duel/split antennas and the fork tongue serpent on the back sides. Any info on this object is appreciated. If privacy is an issue email: chaosonline7@gmail.com (less)

Duration : 0:2:17

These strange microscopic objects were found in skin lesions of a person who suffers from both Lyme Disease and Morgellons. She also lives directly under a flight path of a major airport and near a 2001 hazmat event involving a (glycol) spill that required bioremediation.

I will be posting a few additional videos showing the objects have moving components (possibly nano) and eject a translucent hexagon from a crystal like cup. Please note the duel/split antennas and the fork tongue serpent on the back sides. Any info on this object is appreciated. If privacy is an issue email: chaosonline7@gmail.com

Duration : 0:1:17

I will be posting a few additional videos showing the objects glow (mirrors?) when in contact with metal; have moving components (possibly nano) and eject a translucent hexagon from a crystal like cup. Please note the duel/split antennas and the fork tongue serpent on the back sides. Any info on this object is appreciated. If privacy is an issue email: chaosonline7@gmail.com

Duration : 0:3:28

Duration : 0:9:57

Duration : 0:9:56