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DNA Hydridization Fluidics Movie

DNA Hybridization CD:

The final step in nucleic acid analysis is detection. Fluorescence is often used for detection, and specifically fluorescence microarrays are used when scanning for many elements at once. The Madou BioMEMS group had developed a modular, microfluidic DNA microarray/hybridization CD that allows for rapid hybridization and detection. DNA probes are spotted onto a glass slide using traditional methods, and a microfluidic PDMS unit is then bound passively to the slide. The slide-PDMS unit is then mounted in a CD holder. The sample is sent across the probe array, followed by a wash and then rinse. The entire process takes < 15 mins, a drastic improvement over traditional passive arrays. Finally, the PDMS unit is removed and the slide is scanned in a traditional fluorescence scanner. Superior signal to noise ratios are achieved, and specificity is excellent.


Peytavi, R. (2005). Microfluidic Device for Rapid (< 15 min) Automated Microarray Hybridization. Clinical Chemistry, 51(10), 1836-.

Jia, G. (2006). Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform. Sensors and actuators. B, Chemical, 114(1), 173-.

Duration : 0:1:12

Filed Under: Bio-MEMS


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